Plate Count
The plate height given as:
with the column length and : the number of theoretical plates can be estimated from a chromatogram by analysis of the retention time for each component and its standard deviation as a measure for peak width, provided that the elution curve represents a Gaussian curve.
In this case the plate count is given by:
By using the more practical peak width at half height the equation is:
or with the width at the base of the peak:
Read more about this topic: Van Deemter Equation
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