Examples
- See the main article on list of restriction enzyme cutting sites.
Examples of restriction enzymes include:
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli | 5'GAATTC 3'CTTAAG | 5'---G AATTC---3' 3'---CTTAA G---5' |
| EcoRII | Escherichia coli | 5'CCWGG 3'GGWCC | 5'--- CCWGG---3' 3'---GGWCC ---5' |
| BamHI | Bacillus amyloliquefaciens | 5'GGATCC 3'CCTAGG | 5'---G GATCC---3' 3'---CCTAG G---5' |
| HindIII | Haemophilus influenzae | 5'AAGCTT 3'TTCGAA | 5'---A AGCTT---3' 3'---TTCGA A---5' |
| TaqI | Thermus aquaticus | 5'TCGA 3'AGCT | 5'---T CGA---3' 3'---AGC T---5' |
| NotI | Nocardia otitidis | 5'GCGGCCGC 3'CGCCGGCG | 5'---GC GGCCGC---3' 3'---CGCCGG CG---5' |
| HinfI | Haemophilus influenzae | 5'GANTCA 3'CTNAGT | 5'---G ANTC---3' 3'---CTNA G---5' |
| Sau3A | Staphylococcus aureus | 5'GATC 3'CTAG | 5'--- GATC---3' 3'---CTAG ---5' |
| PvuII* | Proteus vulgaris | 5'CAGCTG 3'GTCGAC | 5'---CAG CTG---3' 3'---GTC GAC---5' |
| SmaI* | Serratia marcescens | 5'CCCGGG 3'GGGCCC | 5'---CCC GGG---3' 3'---GGG CCC---5' |
| HaeIII* | Haemophilus aegyptius | 5'GGCC 3'CCGG | 5'---GG CC---3' 3'---CC GG---5' |
| HgaI | Haemophilus gallinarum | 5'GACGC 3'CTGCG | 5'---NN NN---3' 3'---NN NN---5' |
| AluI* | Arthrobacter luteus | 5'AGCT 3'TCGA | 5'---AG CT---3' 3'---TC GA---5' |
| EcoRV* | Escherichia coli | 5'GATATC 3'CTATAG | 5'---GAT ATC---3' 3'---CTA TAG---5' |
| EcoP15I | Escherichia coli | 5'CAGCAGN25NN 3'GTCGTCN25NN | 5'---CAGCAGN25NN ---3' 3'---GTCGTCN25 NN---5' |
| KpnI | Klebsiella pneumoniae | 5'GGTACC 3'CCATGG | 5'---GGTAC C---3' 3'---C CATGG---5' |
| PstI | Providencia stuartii | 5'CTGCAG 3'GACGTC | 5'---CTGCA G---3' 3'---G ACGTC---5' |
| SacI | Streptomyces achromogenes | 5'GAGCTC 3'CTCGAG | 5'---GAGCT C---3' 3'---C TCGAG---5' |
| SalI | Streptomyces albus | 5'GTCGAC 3'CAGCTG | 5'---G TCGAC---3' 3'---CAGCT G---5' |
| ScaI | Streptomyces caespitosus | 5'AGTACT 3'TCATGA | 5'---AGT ACT---3' 3'---TCA TGA---5' |
| SpeI | Sphaerotilus natans | 5'ACTAGT 3'TGATCA | 5'---A CTAGT---3' 3'---TGATC A---5' |
| SphI | Streptomyces phaeochromogenes | 5'GCATGC 3'CGTACG | 5'---GCATG C---3' 3'---C GTACG---5' |
| StuI | Streptomyces tubercidicus | 5'AGGCCT 3'TCCGGA | 5'---AGG CCT---3' 3'---TCC GGA---5' |
| XbaI | Xanthomonas badrii | 5'TCTAGA 3'AGATCT | 5'---T CTAGA---3' 3'---AGATC T---5' |
Key:
* = blunt ends
N = C or G or T or A
W = A or T
Read more about this topic: Restriction Enzyme
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