Haemophilus

ire at least one of the following blood factors for growth: hemin (factor X) and/or nicotinamide adenine dinucleotide (factor V). Chocolate agar is an excellent Haemophilus growth medium as it allows for increased accessibility to these factors. Alternatively, Haemophilus is sometimes cultured using the "Staph streak" technique: both Staphylococcus and Haemophilus organisms are cultured together on a single blood agar plate. In this case, Haemophilus colonies will frequently grow in small "satellite" colonies around the larger Staphylococcus colonies because the metabolism of Staphylococcus produces the necessary blood factor by-products required for Haemophilus growth.