Quantitative Parasitology - How To Describe The Parasitic Infection of A Sample of Hosts

How To Describe The Parasitic Infection of A Sample of Hosts

Always give the host sample size. In most cases, this is expressed as the number of hosts individuals examined. (Exceptionally, other units may also be used for special cases.)

Describe prevalence. This is the proportion of infected hosts among all the hosts examined. Give the confidence interval (CI) of prevalence (either as a Clopper-Pearson interval or as adjusted Wald/Sterne's interval) to indicate the accuracy of the estimation (use of the confidence intervals belonging to the 95% probability is advisable).

Describe mean intensity. This is the mean number of parasites found in the infected hosts (the zeros of uninfected hosts are excluded). Since sample size and prevalence are known, mean intensity defines the quantity of parasites found in the sample of hosts. Given the typical aggregated (right-skewed) distribution of parasites, its actual value is highly dependent on a few extremely infected hosts. Also give CI to indicate the accuracy of the estimation. Use bias-corrected and accelerated bootstrap (BCa Bootstrap) to get this confidence interval.

Describe median intensity. This is the median number of parasites found in infected hosts (the zeros of uninfected hosts are excluded). Median intensity shows a typical level of infection among the infected hosts. Use exact CI to indicate the accuracy of the estimation.

In certain cases one may prefer to use mean abundance instead of mean intensity. This is the mean number of parasites found in all hosts (involves the zero values of uninfected hosts). Give BCa Bootstrap confidence interval to indicate the accuracy of this estimation. This measure unifies two of the former ones: prevalence and mean intensity. Do not use it, unless you have a clearly specified a reason why to prefer it.

Describing mean crowding (intensity values averaged across parasite individuals) and its confidence interval is essential only for those who study density-dependent characters of parasites. BCa Bootstrap CI can be used to indicate the accuracy of the estimation.

Finally, quantify levels of skewness of the parasites' distribution among hosts. There are 3 indices widely used for this purpose, but their interpretation is quite similar. They predict each other rather well, thus it is not necessary to use all the 3 of them.

Read more about this topic:  Quantitative Parasitology

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