Particle-induced X-ray Emission - Protein Analysis

Protein Analysis

Protein analysis using microPIXE allow for the determination of the elemental composition of liquid and crystalline proteins. microPIXE can quantify the metal content of protein molecules with a relative accuracy of between 10% and 20%.

The advantage of microPIXE is that given a protein of known sequence, the X-ray emission from sulfur can be used as an internal standard to calculate the number of metal atom per protein monomer. Because only relative concentrations are calculated there are only minimal systematic errors, and the results are totally internally consistent.

The relative concentrations of DNA to protein (and metals) can also be measured using the phosphate groups of the bases as an internal calibration.

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