Procedures
- Wet each disk with about four inoculating loops of deionized water.
- Use a loop to aseptically transfer a large mass of pure bacteria to the disk.
- Observe the disk for up to three minutes. If the area of inoculation turns dark-blue to maroon to almost black, then the result is positive. If a color change does not occur within three minutes, the result is negative.
In alternative manner, live bacteria cultivated on trypticase soy agar plates may be prepared using sterile technique with a single-line streak inoculation. The inoculated plates are incubated at 37°C for 24–48 hours to establish colonies. Fresh bacterial preparations should be used. After colonies have grown on the medium, 2-3 drops of the reagent DMPD are added to the surface of each organism to be tested.
- A positive test (OX+) will result in a color change to pink, through maroon and into black, within 10–30 seconds.
- A negative test (OX-) will result in a light-pink or absence of coloration.
Read more about this topic: Oxidase Test
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