Culture Conditions
In general, any glucose-containing medium is suitable for the culture and counting of yeasts, e.g. Sabouraud medium, malt extract agar (MEA), tryptone glucose yeast extract agar (TGY), yeast glucose chloramphenicol agar (YGC). For the detection of acid-resistant yeasts like Z. bailii, acidified media are recommended, such as MEA or TGY with 0.5% (v/v) acetic acid added. Plating with agar media is often used for counting of yeasts, with surface spreading technique is preferable to pour plate method because the former technique gives a better recovery of cells with lower dilution errors. The common incubation conditions are aerobic atmosphere, temperature 25°C for a period of 5 days. Nevertheless, a higher incubation temperature (30°C) and shorter incubation time (3 days) can be applied for Z. bailii, as the yeast grows faster at this elevated temperature.
Read more about this topic: Zygosaccharomyces Bailii
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