Culture Conditions
In general, any glucose-containing medium is suitable for the culture and counting of yeasts, e.g. Sabouraud medium, malt extract agar (MEA), tryptone glucose yeast extract agar (TGY), yeast glucose chloramphenicol agar (YGC). For the detection of acid-resistant yeasts like Z. bailii, acidified media are recommended, such as MEA or TGY with 0.5% (v/v) acetic acid added. Plating with agar media is often used for counting of yeasts, with surface spreading technique is preferable to pour plate method because the former technique gives a better recovery of cells with lower dilution errors. The common incubation conditions are aerobic atmosphere, temperature 25°C for a period of 5 days. Nevertheless, a higher incubation temperature (30°C) and shorter incubation time (3 days) can be applied for Z. bailii, as the yeast grows faster at this elevated temperature.
Read more about this topic: Zygosaccharomyces Bailii
Famous quotes containing the words culture and/or conditions:
“Letting a hundred flowers blossom and a hundred schools of thought contend is the policy for promoting the progress of the arts and the sciences and a flourishing culture in our land.”
—Mao Zedong (1893–1976)
“What is Americanism? Every one has a different answer. Some people say it is never to submit to the dictation of a King. Others say Americanism is the pride of liberty and the defence of an insult to the flag with their gore. When some half-developed person tramples on that flag, we should be ready to pour out the blood of the nation, they say. But do we not sit in silence when that flag waves over living conditions which should be an insult to all patriotism?”
—Anna Howard Shaw (1847–1919)