Migration Speed
In 1% agarose gels, xylene cyanol typically migrates at about the same rate as a 4000 base pair DNA fragment. Xylene cyanol or tracking dye on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment. On 20% denaturating (7 M urea) polyacrylamide gel electrophoresis (PAGE) xylene cyanol migrates at about rate of 25 base oligonucleotide. Typically 0.005-0.03% of final concentration of xylene cyanol is used.
Read more about this topic: Xylene Cyanol
Famous quotes containing the word speed:
“It was undoubtedly the feeling of exile—that sensation of a void within which never left us, that irrational longing to hark back to the past or else to speed up the march of time, and those keen shafts of memory that stung like fire.”
—Albert Camus (1913–1960)