William Johnston may refer to:
- William Johnston (judge) (1804-1891), judge from Ohio
- William Johnston (congressman) (1819–1866), U.S. Representative from Ohio
- William Johnston (Irish politician) (1829–1902), Irish politician and Orangeman
- William Johnston of Liverpool, early benefactor of the University of Liverpool, Liverpool, England
- William Ernest George Johnston (1884–1951), Lord Mayor of Belfast
- William F. Johnston (1808–1872), governor of Pennsylvania, 1848–1852
- William N. Johnston, 16th president of Wesley College (Delaware)
- William Henry Johnston (1879–1915), Scottish recipient of the Victoria Cross
- William J. Johnston (1918–1990), American soldier and Medal of Honor recipient
- Willie Johnston (Medal of Honor) (1850–?)
- William Johnston (bishop) (1914–1986), of Dunwich
- Billy Johnston (footballer born 1901), Scottish former footballer
- Willie Johnston (born 1946), Scottish former footballer
- Delirious (wrestler) (William Hunter Johnston, born 1981), professional wrestler
- William Johnston (Canadian politician) (1876–1925), Labour MLA for Medicine Hat, 1921–1926
- Massa Johnston (William Johnston, 1881–1951), New Zealand rugby union and rugby league representative
- William L. Johnston (1811–1849), carpenter-architect in Philadelphia
- William Preston Johnston (1831–1899), lawyer, scholar, poet, and Confederate soldier
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