Medical Diagnostic Applications
- The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. Proteins from known HIV-infected cells are separated and blotted on a membrane as above. Then, the serum to be tested is applied in the primary antibody incubation step; free antibody is washed away, and a secondary anti-human antibody linked to an enzyme signal is added. The stained bands then indicate the proteins to which the patient's serum contains antibody.
- A western blot is also used as the definitive test for Bovine spongiform encephalopathy (BSE, commonly referred to as 'mad cow disease').
- Some forms of Lyme disease testing employ western blotting.
- Western blot can also be used as a confirmatory test for Hepatitis B infection.
- In veterinary medicine, western blot is sometimes used to confirm FIV+ status in cats.
Read more about this topic: Western Blot
Famous quotes containing the word medical:
“Every day our garments become more assimilated to ourselves, receiving the impress of the wearer’s character, until we hesitate to lay them aside without such delay and medical appliances and some such solemnity even as our bodies.”
—Henry David Thoreau (1817–1862)