64-bit Support
Support for Virtual 8086 mode is not available in x86-64 Long Mode, although it is still present on 64-bit capable processors running in 32-bit protected mode. The removal of this sub-mode means that 16-bit compatibility must be rewritten or removed from 64-bit operating systems. For example, DOS and 16-bit Windows application support is not present in x64 editions of Windows, necessitating the use of 3rd party emulation software such as DOSBox.
Read more about this topic: Virtual 8086 Mode
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