U.S. Route 301

U.S. Route 301 is a spur of U.S. Route 1 running through the South Atlantic States. It currently runs 1,099 miles (1,769 km) from Glasgow, Delaware at U.S. Route 40 to Sarasota, Florida. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, Brandon, and Sarasota, Florida.

US 301 parallels Interstate 95 for much of its routing through The Carolinas and Virginia including a short concurrency in Santee, South Carolina and Lumberton, North Carolina.

US 301 has a number of concurrencies along its route. It overlaps U.S. Route 50 from Bowie, Maryland to Queenstown, Maryland, including the Chesapeake Bay Bridge; with unsigned Interstate 595 from Bowie, Maryland to Annapolis, Maryland; and Maryland Route 5 from Brandywine, Maryland to Waldorf, Maryland among others.

US 301 formerly had its terminus in Baltimore, Maryland. It followed the alignment of the current Maryland Route 3, portions of the Baltimore Beltway, and Maryland Route 648. Route 301 ended in Southwestern Baltimore on Monroe Street at the intersection with U.S. 1. Route 3 was supplanted north of Millersville by Interstate 97.