Result
Hybridization of the probe to a specific DNA fragment on the filter membrane indicates that this fragment contains DNA sequence that is complementary to the probe.
The transfer step of the DNA from the electrophoresis gel to a membrane permits easy binding of the labeled hybridization probe to the size-fractionated DNA. It also allows for the fixation of the target-probe hybrids, required for analysis by autoradiography or other detection methods.
Southern blots performed with restriction enzyme-digested genomic DNA may be used to determine the number of sequences (e.g., gene copies) in a genome. A probe that hybridizes only to a single DNA segment that has not been cut by the restriction enzyme will produce a single band on a Southern blot, whereas multiple bands will likely be observed when the probe hybridizes to several highly similar sequences (e.g., those that may be the result of sequence duplication). Modification of the hybridization conditions (for example, increasing the hybridization temperature or decreasing salt concentration) may be used to increase specificity and decrease hybridization of the probe to sequences that are less than 100% similar.
Read more about this topic: Southern Blot
Famous quotes containing the word result:
“A true politeness does not result from any hasty and artificial polishing, it is true, but grows naturally in characters of the right grain and quality, through a long fronting of men and events, and rubbing on good and bad fortune.”
—Henry David Thoreau (1817–1862)
“The superficiality of the American is the result of his hustling. It needs leisure to think things out; it needs leisure to mature. People in a hurry cannot think, cannot grow, nor can they decay. They are preserved in a state of perpetual puerility.”
—Eric Hoffer (1902–1983)
“The whole [of religion] is a riddle, an ænigma, an inexplicable mystery. Doubt, uncertainty, suspence of judgment appear the only result of our most accurate scrutiny, concerning this subject.”
—David Hume (1711–1776)