Result
Hybridization of the probe to a specific DNA fragment on the filter membrane indicates that this fragment contains DNA sequence that is complementary to the probe.
The transfer step of the DNA from the electrophoresis gel to a membrane permits easy binding of the labeled hybridization probe to the size-fractionated DNA. It also allows for the fixation of the target-probe hybrids, required for analysis by autoradiography or other detection methods.
Southern blots performed with restriction enzyme-digested genomic DNA may be used to determine the number of sequences (e.g., gene copies) in a genome. A probe that hybridizes only to a single DNA segment that has not been cut by the restriction enzyme will produce a single band on a Southern blot, whereas multiple bands will likely be observed when the probe hybridizes to several highly similar sequences (e.g., those that may be the result of sequence duplication). Modification of the hybridization conditions (for example, increasing the hybridization temperature or decreasing salt concentration) may be used to increase specificity and decrease hybridization of the probe to sequences that are less than 100% similar.
Read more about this topic: Southern Blot
Famous quotes containing the word result:
“It is the custom of the immortal gods to grant temporary prosperity and a fairly long period of impunity to those whom they plan to punish for their crimes, so that they may feel it all the more keenly as a result of the change in their fortunes.”
—Julius Caesar [Gaius Julius Caesar] (100–44 B.C.)
“We shall make mistakes, but they must never be mistakes which result from faintness of heart or abandonment of moral principles. I remember that my old school master Dr. Peabody said in days that seemed to us then to be secure and untroubled, he said things in life will not always run smoothly, sometimes we will be rising toward the heights and all will seem to reverse itself and start downward. The great thing to remember is that the trend of civilization itself is forever upward.”
—Franklin D. Roosevelt (1882–1945)
“Vanity is as advantageous to a government as pride is dangerous. To be convinced of this we need only represent, on the one hand, the numberless benefits which result from vanity, as industry, the arts, fashions, politeness, and taste; and on the other, the infinite evils which spring from the pride of certain nations, a laziness, poverty, a total neglect of everything.”
—Charles Louis de Secondat Montesquieu (1689–1755)