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- Río Negro (newspaper), published in Argentina
- Atlético Rio Negro Clube, a Brazilian football team from Manaus, Amazonas state
- Rionegro (TransMilenio) a TransMilenio transportation system station in Bogotá, Colombia
- Río Negro Massacre, Guatemala
- Rio Negro tuco-tuco, a species of rodent
- Rio Negro Brush-tailed Rat, a species of rodent
- Palácio Rio Negro, one of the official residences of the President of Brazil
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