Expression of Recombinant DNA
Following transplantation into the host organism, the foreign DNA contained within the recombinant DNA construct may or may not be expressed. That is, the DNA may simply be replicated without expression, or it may be transcribed and translated so that a recombinant protein is produced. Generally speaking, expression of a foreign gene requires restructuring the gene to include sequences that are required for producing a mRNA molecule that can be used by the host's translational apparatus (e.g. promoter, translational initiation signal, and transcriptional terminator). Specific changes to the host organism may be made to improve expression of the ectopic gene. In addition, changes may be needed to the coding sequences as well, to optimize translation, make the protein soluble, direct the recombinant protein to the proper cellular or extracellular location, and stabilize the protein from degradation.
Read more about this topic: Recombinant DNA
Famous quotes containing the words expression of, expression and/or dna:
“No compliment can be eloquent, except as an expression of indifference.”
—George Eliot [Mary Ann (or Marian)
“As a man has no right to kill one of his children if it is diseased or insane, so a man who has made the gradual and conscious expression of his personality in literature the aim of his life, has no right to suppress himself any carefully considered work which seemed good enough when it was written. Suppression, if it is deserved, will come rapidly enough from the same causes that suppress the unworthy members of a man’s family.”
—J.M. (John Millington)
“Here [in London, history] ... seemed the very fabric of things, as if the city were a single growth of stone and brick, uncounted strata of message and meaning, age upon age, generated over the centuries to the dictates of some now all-but-unreadable DNA of commerce and empire.”
—William Gibson (b. 1948)