Recombinant DNA - Expression of Recombinant DNA

Expression of Recombinant DNA

Following transplantation into the host organism, the foreign DNA contained within the recombinant DNA construct may or may not be expressed. That is, the DNA may simply be replicated without expression, or it may be transcribed and translated so that a recombinant protein is produced. Generally speaking, expression of a foreign gene requires restructuring the gene to include sequences that are required for producing a mRNA molecule that can be used by the host's translational apparatus (e.g. promoter, translational initiation signal, and transcriptional terminator). Specific changes to the host organism may be made to improve expression of the ectopic gene. In addition, changes may be needed to the coding sequences as well, to optimize translation, make the protein soluble, direct the recombinant protein to the proper cellular or extracellular location, and stabilize the protein from degradation.

Read more about this topic:  Recombinant DNA

Famous quotes containing the words expression of, expression and/or dna:

    Surely it is one of the requisites of a tasteful garb that the expression of effort to please shall be wanting in it; that the mysteries of the toilet shall not be suggested by it; that the steps to its completion shall be knocked away like the sculptor’s ladder from the statue, and the mental force expended upon it be swept away out of sight like the chips on the studio floor.
    Elizabeth Stuart Phelps (1844–1911)

    Every production of an artist should be the expression of an adventure of his soul.
    W. Somerset Maugham (1874–1965)

    Here [in London, history] ... seemed the very fabric of things, as if the city were a single growth of stone and brick, uncounted strata of message and meaning, age upon age, generated over the centuries to the dictates of some now all-but-unreadable DNA of commerce and empire.
    William Gibson (b. 1948)