Example
Consider an experiment with a treatment group and one control group. Suppose the experimenter has recruited a population of 50 people for the experiment—25 with blue eyes and 25 with brown eyes. If the experimenter were to assign all of the blue-eyed people to the treatment group and the brown-eyed people to the control group, the results may be biased. When analyzing the results, one might question whether an observed effect was due to the application of the experimental condition or was in fact due to eye color.
With random assignment, one would randomly assign individuals to either treatment or control and therefore have a better chance at detecting if an observed change is due to chance or due to the experimental treatment.
If a randomly assigned group is compared to the mean it may be discovered that statistically they differ, even though they were assigned from the same group. To express this same idea statistically - If a test of statistical significance is applied to randomly assigned groups to test the difference between sample means against the null hypothesis that they are equal to the same population mean (i.e., population mean of differences = 0), given the probability distribution, the null hypothesis will sometimes be "rejected," that is, deemed not plausible. That is, the groups will be sufficiently different on the variable tested to conclude statistically that they did not come from the same population, even though, procedurally, they were assigned from the same total group. In the example above using random assignment may create an assignment to groups that has 20 blue-eyed people and 5 brown-eyed people in one group. This is a rare event under random assignment, but it could happen, and when it does it might add some doubt to the causal agent in the experimental hypothesis.
Because most basic statistical tests require the hypothesis of an independent randomly sampled population, random assignment is the desired assignment method because it provides control for all attributes of the members of the samples—in contrast to matching on only one or more variables—and provides the mathematical basis for estimating the likelihood of group equivalence for characteristics one is interested in, both for pretreatment checks on equivalence and the evaluation of post treatment results using inferential statistics. More advanced statistical modeling can be used to adapt the inference to the sampling method.
Read more about this topic: Random Assignment
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