Identification of Relative Location
As promoters are typically immediately adjacent to the gene in question, positions in the promoter are designated relative to the transcriptional start site, where transcription of DNA begins for a particular gene (i.e., positions upstream are negative numbers counting back from -1, for example -100 is a position 100 base pairs upstream).
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“Man may have his opinion as to the relative importance of feeding his body and nourishing his soul, but he is allowed by Nature to have no opinion whatever as to the need for feeding the body before the soul can think of anything but the body’s hunger.”
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