Ovarian Follicle - Cryopreservation and Culture

Cryopreservation and Culture

Follicles can develop from ovarian tissue after cryopreservation. Cryopreservation of ovarian tissue is of interest to women who want to preserve their reproductive function beyond the natural limit, or whose reproductive potential is threatened by cancer therapy, for example in hematologic malignancies or breast cancer.

For in vitro culture of follicles, there are various techniques to optimize the growth of follicles, including the use of defined media, growth factors and three-dimensional extracellular matrix support. Molecular methods and immunoassay can evaluate stage of maturation and guide adequate differentiation.