Margaret Beaufort may refer to:
- Margaret Beaufort, Countess of Richmond and Derby (1443–1509), the daughter of John Beaufort, 1st Duke of Somerset (first creation) and the mother of Henry VII of England. Also known as Lady Stanley.
- Margaret Beaufort, Countess of Stafford (c. 1427–1474), the daughter of Edmund Beaufort, 1st Duke of Somerset (second creation) and the mother of Henry Stafford, 2nd Duke of Buckingham.
- Margaret Beaufort, Countess of Devon (c. 1409–1449), the daughter of John Beaufort, 1st Earl of Somerset, the mother of both Thomas Courtenay, 14th Earl of Devon and John Courtenay, 15th Earl of Devon.
- A school in the village of Riseley, Bedfordshire, named after the Countess of Richmond.