Research
Because the 5' → 3' exonuclease activity of DNA polymerase I from E. coli makes it unsuitable for many applications, the Klenow fragment, which lacks this activity, can be very useful in research. The Klenow fragment is extremely useful for research-based tasks such as:
- Synthesis of double-stranded DNA from single-stranded templates
- Filling in (meaning removal of overhangs to create blunt ends) recessed 3' ends of DNA fragments
- Digesting away protruding 3' overhangs
- Preparation of radioactive DNA probes
The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase.
Read more about this topic: Klenow Fragment
Famous quotes containing the word research:
“... research is never completed ... Around the corner lurks another possibility of interview, another book to read, a courthouse to explore, a document to verify.”
—Catherine Drinker Bowen (1897–1973)
“The working woman may be quick to see any problems with children as her fault because she isn’t as available to them. However, the fact that she is employed is rarely central to the conflict. And overall, studies show, being employed doesn’t have negative effects on children; carefully done research consistently makes this clear.”
—Grace Baruch (20th century)
“The research on gender and morality shows that women and men looked at the world through very different moral frameworks. Men tend to think in terms of “justice” or absolute “right and wrong,” while women define morality through the filter of how relationships will be affected. Given these basic differences, why would men and women suddenly agree about disciplining children?”
—Ron Taffel (20th century)