A killing jar is a device used by entomologists to kill captured insects quickly and with minimum damage. The jar, typically glass, must be hermetically sealable and one design has a thin layer of hardened plaster of paris on the bottom to absorb the killing agent. The killing agent will then slowly evaporate, allowing the jar to be used many times before needing to refresh the jar. The absorbent plaster of paris layer also helps prevent the agent sticking to and damaging insects. Crumpled paper tissue is also placed in the jar for the same reason. A second method utilises a wad of cotton or other absorbent material placed in the bottom of the jar. Liquid killing agent is then added until the absorbent material is nearly saturated. A piece of stiff paper or cardboard cut to fit the inside of the jar tightly is then pressed in. Another design is shown on the right. This has to be recharged more frequently.
The most common killing agents are ether, chloroform and ethyl acetate. Ethyl acetate has many advantages and is very widely used. Its fumes are less toxic to humans than those of the other agents and specimens will remain limp if they are left in an ethyl acetate killing jar for several days and the ethyl acetate is not allowed to entirely evaporate from the specimens. A disadvantage is that although the insects are quickly stunned by ethyl acetate it kills them slowly and specimens may revive if removed from the killing jar too soon. Potassium cyanide or other cyanide compounds are also used, but only by experts due to its extreme toxicity. The potassium cyanide slowly decomposes, releasing hydrogen cyanide.
Killing jars are only used on hard-bodied insects. Soft-bodied insects, such as the larval stage, are generally fixed in ethanol at 70-80% concentration.
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