Joseph Wolstenholme (September 30, 1829 – November 18, 1891) was an English mathematician.
Wolstenholme was born in Eccles near Salford, Lancashire, England. He graduated from St John's College, Cambridge as Third Wrangler in 1850 and was elected a fellow of Christ's College in 1852. He became a professor of mathematics at the Royal Indian Engineering College at Cooper's Hill, Egham near London from 1871 to 1889, and was the author of Mathematical problems.
He was a close friend of Leslie Stephen from his undergraduate studies at Cambridge. Virginia Woolf used his personality for the character Augustus Carmichael in her novel To the Lighthouse. His sister was the feminist Elizabeth Clarke Wolstenholme Elmy.