John Knightley may refer to:
- Emma#Principal characters
- John Knightley (MP) for Warwickshire (UK Parliament constituency)
- Sir John Knightley, 1st Baronet (c. 1611–c. 1670) of the Knightley Baronets
- Sir John Knightley, 2nd Baronet (d. 1689) of the Knightley Baronets
- Sir John Knightley, 1st Baronet (1747–1812) of the Knightley Baronets