John Banister may refer to:
- John Banister (anatomist) (1533–1610), English anatomist
- John Banister (composer) (c. 1624–1679), English composer
- John Banister (naturalist) (1650–1692), English clergyman and natural scientist
- John Banister (lawyer) (1734–1788), American delegate in the Continental Congress
- John Riley Banister (1854–1918), American law officer and Texas Ranger