Current Problems With The Isolation of Proteins From Bacterial Inclusion Bodies
70-80% of recombinant proteins expressed E. coli are contained in inclusion bodies (i.e., protein aggregates). The purification of the expressed proteins from inclusion bodies usually require two main steps: extraction of inclusion bodies from the bacteria followed by the solubilisation of the purified inclusion bodies. This is considered labour-intensive, time consuming and not cost effective.
Read more about this topic: Inclusion Body
Famous quotes containing the words current, problems, isolation, proteins, inclusion and/or bodies:
“The current of our thoughts made as sudden bends as the river, which was continually opening new prospects to the east or south, but we are aware that rivers flow most rapidly and shallowest at these points.”
—Henry David Thoreau (18171862)
“Im no good at being noble, but it doesnt take much to see that the problems of three little people dont amount to a hill of beans in this crazy world. Someday youll understand that.”
—Julius J. Epstein, U.S. screenwriter, Philip Epstein, screenwriter, Howard Koch, screenwriter, and Michael Curtiz. Rick Blaine (Humphrey Bogart)
“But, my dear, you cannot live in isolation from the human race, you know.”
—John Clifford, U.S. screenwriter, and Herk Harvey. Minister (Stan Levitt)
“Civilization means food and literature all round. Beefsteaks and fiction magazines for all. First-class proteins for the body, fourth-class love-stories for the spirit.”
—Aldous Huxley (18941963)
“Belonging to a group can provide the child with a variety of resources that an individual friendship often cannota sense of collective participation, experience with organizational roles, and group support in the enterprise of growing up. Groups also pose for the child some of the most acute problems of social lifeof inclusion and exclusion, conformity and independence.”
—Zick Rubin (20th century)
“When the landscape buckles and jerks around, when a dust column of debris rises from the collapse of a block of buildings on bodies that could have been your own, when the staves of history fall awry and the barrel of time bursts apart, some turn to prayer, some to poetry: words in the memory, a stained book carried close to the body, the notebook scribbled by handa center of gravity.”
—Adrienne Rich (b. 1929)