Haemophilus Influenzae - Sequencing

Sequencing

H. influenzae was the first free-living organism to have its entire genome sequenced. Completed by Craig Venter and his team, Haemophilus was chosen because one of the project leaders, Nobel laureate Hamilton Smith, had been working on it for decades and was able to provide high-quality DNA libraries. The genome consists of 1,830,140 base pairs of DNA in a single circular chromosome that contains 1740 protein-coding genes, 58 transfer RNA genes, and 18 other RNA genes. The sequencing method used was whole-genome shotgun, which was completed and published in Science in 1995 and conducted at The Institute for Genomic Research.