Conclusions
Expression profiling provides new information about what genes do under various conditions. Overall, microarray technology produces reliable expression profiles. From this information one can generate new hypotheses about biology or test existing ones. However, the size and complexity of these experiments often results in a wide variety of possible interpretations. In many cases, analyzing expression profiling results takes far more effort than performing the initial experiments.
Most researchers use multiple statistical methods and exploratory data analysis before publishing their expression profiling results, coordinating their efforts with a bioinformatician or other expert in microarray technology. Good experimental design, adequate biological replication and follow up experiments play key roles in successful expression profiling experiments.
Read more about this topic: Gene Expression Profiling
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