Gene Expression - Techniques and Tools

Techniques and Tools

The following experimental techniques are used to measure gene expression and are listed in roughly chronological order, starting with the older, more established technologies. They are divided into two groups based on their degree of multiplexity.

  • Low-to-mid-plex techniques:
    • Reporter gene
    • Northern blot
    • Western blot
    • Fluorescent in situ hybridization
    • Reverse transcription PCR
  • Higher-plex techniques:
    • SAGE
    • DNA microarray
    • Tiling array
    • RNA-Seq

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