Frozen Sections
Small pieces of tissue (5×5×3mm) are placed in a cryoprotective embedding medium—OCT, TBS, or Cryogel—then snap frozen in isopentane cooled by liquid nitrogen. Tissue is then sectioned in a freezing microtome or cryostat. Sections are then fixed in one of the following fixatives: Absolute acetone for 10–15 minutes, 95% ethanol for 10–15 minutes or Absolute acetone 10 minutes followed by 95% ethanol 10 minutes
Read more about this topic: Fixation (histology)
Famous quotes containing the words frozen and/or sections:
“Who are we? And for what are we going to fight? Are we the titled slaves of George the Third? The military conscripts of Napoleon the Great? Or the frozen peasants of the Russian Czar? No—we are the free born sons of America; the citizens of the only republic now existing in the world; and the only people on earth who possess rights, liberties, and property which they dare call their own.”
—Andrew Jackson (1767–1845)
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—Eleanor Roosevelt (1884–1962)