Frozen Sections
Small pieces of tissue (5×5×3mm) are placed in a cryoprotective embedding medium—OCT, TBS, or Cryogel—then snap frozen in isopentane cooled by liquid nitrogen. Tissue is then sectioned in a freezing microtome or cryostat. Sections are then fixed in one of the following fixatives: Absolute acetone for 10–15 minutes, 95% ethanol for 10–15 minutes or Absolute acetone 10 minutes followed by 95% ethanol 10 minutes
Read more about this topic: Fixation (histology)
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