Electron Microscope

An electron microscope uses condensing lenses to focus a beam of electrons to illuminate a specimen and produce a magnified image. An electron microscope (EM) has greater resolving power than a light-powered optical microscope because electrons have wavelengths about 100,000 times shorter than visible light photons. They can achieve better than 50 pm resolution and magnifications of up to about 10,000,000x whereas ordinary, non-confocal light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000x.

The electron microscope uses electrostatic and electromagnetic "lenses" to control the electron beam and focus it to form an image. These lenses are analogous to but different from the glass lenses of an optical microscope that form a magnified image by focusing light on or through the specimen. Electron microscopes are used to observe a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, metals, and crystals. Industrially, the electron microscope is often used for quality control and failure analysis. Modern electron microscopes produce electron micrographs, using specialised digital cameras or frame grabbers to capture the image.