Earls of Annandale and Hartfell (1662)
- Subsidiary title: Lord Johnstone (1662)
- James Hope-Johnstone, 3rd Earl of Hopetoun (1741–1816), de jure 5th Earl of Annandale and Hartfell
- Anne Hope-Johnstone (1768–1818) de jure 6th Countess of Annandale and Hartfell
- John James Hope-Johnstone (1796–1876) de jure 7th Earl of Annandale and Hartfell
- John James Hope-Johnstone (1842–1912) de jure 8th Earl of Annandale and Hartfell
- Evelyn Wentworth Hope-Johnstone (1879–1964) de jure 9th Earl of Annandale and Hartfell
- Percy Wentworth Hope-Johnstone (1909–1983) de jure 10th Earl of Annandale and Hartfell
- Patrick Andrew Wentworth Hope-Johnstone, 11th Earl of Annandale and Hartfell (b. 1941) (revived 1985)
The heir apparent is the present holder's son David Patrick Wentworth Hope-Johnstone, Lord Johnstone (b. 1971).
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