Applications of Cell Culture
Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and other products of biotechnology
Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, immunobiologicals (monoclonal antibodies, interleukins, lymphokines), and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are glycosylated (carbohydrate-modified) currently must be made in animal cells. An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants, use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy observation is one of its applications. It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the process.
Read more about this topic: Cell Lines
Famous quotes containing the words cell and/or culture:
“In America all too few blows are struck into flesh. We kill the spirit here, we are experts at that. We use psychic bullets and kill each other cell by cell.”
—Norman Mailer (b. 1923)
“Our culture has become something that is completely and utterly in love with its parent. It’s become a notion of boredom that is bought and sold, where nothing will happen except that people will become more and more terrified of tomorrow, because the new continues to look old, and the old will always look cute.”
—Malcolm McLaren (b. 1946)