Synchronization of Cell Cultures
Several methods can be used to synchronise cell cultures by halting the cell cycle at a particular phase. For example, serum starvation and treatment with thymidine or aphidicolin halt the cell in the G1 phase, mitotic shake-off, treatment with colchicine and treatment with nocodazole halt the cell in M phase and treatment with 5-fluorodeoxyuridine halts the cell in S phase. In addition, partial cell division cycle synchrony can be achieved in budding yeast as a consequence of metabolic synchrony. This metabolic synchrony at the level of synchronized cultures is an emergent, self-organized phenomenon based on a single-cell autonomous cell growth cycle taking place in the G1 phase of the cell division cycle.
Read more about this topic: Cell Cycle
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