Operators
- Chile
- Chilean Air Force operates 3, received its first KC-135E in February 2010.
- France
- French Air Force operates 11 C-135FRs and 3 KC-135s.
- Singapore
- Republic of Singapore Air Force has four tankers, which occasionally function as VIP transport and aeromedical missions.
- Turkey
- Turkish Air Force operates 7 KC-135s.
- United States
- NASA (until 2004)
- United States Air Force operates 417 KC-135s (183 Active Duty, 64 Air Force Reserve, and 170 Air National Guard) as of September 2010.
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- Air Combat Command
- USAF Weapons School - Nellis Air Force Base, Nevada
- 509th Weapons Squadron - Fairchild Air Force Base, Washington
- Air Education and Training Command
- 97th Air Mobility Wing – Altus AFB, Oklahoma
- 54th Air Refueling Squadron
- Air Mobility Command
- 6th Air Mobility Wing – MacDill AFB, Florida
- 91st Air Refueling Squadron
- 99th Air Refueling Squadron – Birmingham IAP/ANGB (Active Associate unit)
- 911th Air Refueling Squadron – Seymour Johnson AFB, North Carolina(Active associate unit)
- 22d Air Refueling Wing – McConnell AFB, Kansas
- 344th Air Refueling Squadron
- 349th Air Refueling Squadron
- 350th Air Refueling Squadron
- 384th Air Refueling Squadron
- 64th Air Refueling Squadron - Pease ANGB, New Hampshire (Active associate unit)
- 92d Air Refueling Wing – Fairchild AFB, Washington
- 92d Air Refueling Squadron
- 93d Air Refueling Squadron
- 912th Air Refueling Squadron - March ARB, California (Active associate unit)
- 375th Air Mobility Wing – Scott AFB, Illinois
- 906th Air Refueling Squadron (Active associate unit)
- Pacific Air Forces
- 18th Wing – Kadena AB, Japan
- 909th Air Refueling Squadron
- United States Air Forces in Europe
- 100th Air Refueling Wing – RAF Mildenhall, England
- 351st Air Refueling Squadron
- Air Force Reserve Command
- 434th Air Refueling Wing – Grissom ARB, Indiana
- 72d Air Refueling Squadron
- 74th Air Refueling Squadron
- 452d Air Mobility Wing – March ARB, California
- 336th Air Refueling Squadron
- 459th Air Refueling Wing – Andrews AFB, Maryland
- 756th Air Refueling Squadron
- 507th Air Refueling Wing – Tinker AFB, Oklahoma
- 18th Air Refueling Squadron – McConnell AFB, Kansas
- 465th Air Refueling Squadron
- 916th Air Refueling Wing – Seymour Johnson AFB, North Carolina
- 77th Air Refueling Squadron
- 927th Air Refueling Wing – MacDill AFB, Florida
- 63d Air Refueling Squadron
- 940th Air Refueling Wing – Beale AFB, California
- 314th Air Refueling Squadron
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- Air National Guard
- Alabama Air National Guard
- 117th Air Refueling Wing – Birmingham IAP/ANGB
- 106th Air Refueling Squadron
- Alaska Air National Guard
- 168th Air Refueling Wing – Eielson AFB
- 168th Air Refueling Squadron
- Arizona Air National Guard
- 161st Air Refueling Wing – Sky Harbor IAP/ANGB
- 197th Air Refueling Squadron
- Hawaii Air National Guard
- 154th Wing – Hickam AFB
- 203d Air Refueling Squadron
- Illinois Air National Guard
- 126th Air Refueling Wing – Scott AFB
- 108th Air Refueling Squadron
- Iowa Air National Guard
- 185th Air Refueling Wing – Sioux Gateway ANGB
- 174th Air Refueling Squadron
- Kansas Air National Guard
- 190th Air Refueling Wing – Forbes Field ANGB
- 117th Air Refueling Squadron
- Maine Air National Guard
- 101st Air Refueling Wing – Bangor IAP/ANGB
- 132d Air Refueilng Squadron
- Michigan Air National Guard
- 127th Wing – Selfridge ANGB
- 171st Air Refueling Squadron
- Mississippi Air National Guard
- 186th Air Refueling Wing – Key Field ANGB
- 153d Air Refueling Squadron
- Nebraska Air National Guard
- 155th Air Refueling Wing – Lincoln ANGB
- 173rd Air Refueling Squadron
- New Hampshire Air National Guard
- 157th Air Refueling Wing – Pease ANGB
- 133d Air Refueling Squadron
- New Jersey Air National Guard
- 108th Air Refueling Wing – McGuire AFB
- 141st Air Refueling Squadron
- 150th Air Refueling Squadron
- Ohio Air National Guard
- 121st Air Refueling Wing – Rickenbacker ANGB
- 145th Air Refueling Squadron
- 166th Air Refueling Squadron
- Pennsylvania Air National Guard
- 171st Air Refueling Wing – Pittsburgh International Airport/Pittsburgh ARS
- 146th Air Refueling Squadron
- 147th Air Refueling Squadron
- Tennessee Air National Guard
- 134th Air Refueling Wing – McGhee Tyson ANGB
- 151st Air Refueling Squadron
- Utah Air National Guard
- 151st Air Refueling Wing – Salt Lake International Airport/ANGB
- Washington Air National Guard
- 141st Air Refueling Wing – Fairchild AFB
- 116th Air Refueling Squadron
- Wisconsin Air National Guard – General Mitchell International Airport
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Note Italy has been reported in some sources as operating several KC-135s, however these are actually Boeing 707-300s converted to tanker configuration.