Bacterial Artificial Chromosome - Common Gene Components

Common Gene Components

oriS, repE - F
for plasmid replication and regulation of copy number.
parA and parB
for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC.
A selectable marker
for antibiotic resistance; some BACs also have lacZ at the cloning site for blue/white selection.
T7 & Sp6
phage promoters for transcription of inserted genes.

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