Common Gene Components
- oriS, repE - F
- for plasmid replication and regulation of copy number.
- parA and parB
- for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC.
- A selectable marker
- for antibiotic resistance; some BACs also have lacZ at the cloning site for blue/white selection.
- T7 & Sp6
- phage promoters for transcription of inserted genes.
Read more about this topic: Bacterial Artificial Chromosome
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