In enzymology, a 5'-acylphosphoadenosine hydrolase (EC 3.6.1.20) is an enzyme that catalyzes the chemical reaction
- 5'-acylphosphoadenosine + H2O AMP + a carboxylate
Thus, the two substrates of this enzyme are 5'-acylphosphoadenosine and H2O, whereas its two products are AMP and carboxylate.
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 5'-acylphosphoadenosine acylhydrolase. This enzyme is also called 5-phosphoadenosine hydrolase. This enzyme participates in purine metabolism.