3,4-dihydroxyphenylacetate 2,3-dioxygenase

In enzymology, a 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15) is an enzyme that catalyzes the chemical reaction

3,4-dihydroxyphenylacetate + O₂ 2-hydroxy-5-carboxymethylmuconate semialdehyde

Thus, the two substrates of this enzyme are 3,4-dihydroxyphenylacetate and O₂, whereas its product is 2-hydroxy-5-carboxymethylmuconate semialdehyde.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O₂. The systematic name of this enzyme class is 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing). Other names in common use include 3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, HPC dioxygenase, and homoprotocatechuate 2,3-dioxygenase. This enzyme participates in tyrosine metabolism. It employs one cofactor, iron.