In enzymology, a 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) is an enzyme that catalyzes the chemical reaction
- (3R)-3-hydroxybutanoyl-CoA crotonoyl-CoA + H2O
Hence, this enzyme has one substrate, (3R)-3-hydroxybutanoyl-CoA, and two products, crotonoyl-CoA and H2O.
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (3R)-3-hydroxybutanoyl-CoA hydro-lyase (crotonoyl-CoA-forming). Other names in common use include D-3-hydroxybutyryl coenzyme A dehydratase, D-3-hydroxybutyryl-CoA dehydratase, enoyl coenzyme A hydrase (D), and (3R)-3-hydroxybutanoyl-CoA hydro-lyase. This enzyme participates in butanoate metabolism.