3-chloro-D-alanine Dehydrochlorinase

In enzymology, a 3-chloro-D-alanine dehydrochlorinase (EC 4.5.1.2) is an enzyme that catalyzes the chemical reaction

3-chloro-D-alanine + H₂O pyruvate + chloride + NH₃

Thus, the two substrates of this enzyme are 3-chloro-D-alanine and H₂O, whereas its 3 products are pyruvate, chloride, and NH₃.

This enzyme belongs to the family of lyases, specifically the class of carbon-halide lyases. The systematic name of this enzyme class is 3-chloro-D-alanine chloride-lyase (deaminating; pyruvate-forming). Other names in common use include beta-chloro-D-alanine dehydrochlorinase, and 3-chloro-D-alanine chloride-lyase (deaminating). It employs one cofactor, pyridoxal phosphate.