Round 2
- (week ending 25 February)
Russian team Kazan Arrows entered the competition. Castleford Lock Lane defeated Bradford Dudley Hill after extra time in the closest game of this round, which against consisted of amateur teams.
| Tie no | Home team | Score | Away team |
|---|---|---|---|
| 1 | Bradford Dudley Hill | 26-28 | Castleford Lock Lane |
| 2 | East Hull | 50-4 | Dewsbury Celtic |
| 3 | Eccles & Salford | 40-28 | Egremont Rangers |
| 4 | Leigh East | 10-25 | Oulton Raiders |
| 5 | Leigh MR | 34-18 | Leeds Metro. University |
| 6 | Rochdale Mayfield | 50-0 | East Leeds |
| 7 | Royal Navy | 26-28 | Normanton |
| 8 | Saddleworth | 21-14 | Siddal |
| 9 | Thornhill | 38-20 | Kazan Arrows |
| 10 | West Hull | 23-16 | Skirlaugh |
| 11 | Widnes St Maries | 22-6 | Hull Isberg |
| 12 | Wigan St Judes | 22-30 | Drighlington |
Read more about this topic: 2007 Challenge Cup