Round 1
- (week ending 4 February)
Teams came from the National Conference League, Rugby League Conference, Student Rugby League, Pennine League, North West Counties, Yorkshire League, CARLA, Hull & District League and the Armed Forces. This round consisted entirely of amateur teams. Fife Lions, the only Scottish club in the tournament, were eliminated.
| Tie no | Home team | Score | Away team |
|---|---|---|---|
| 1 | Rochdale Mayfield | 50-16 | Millom |
| 2 | Saddleworth Rangers | 34-20 | Halton Simms Cross |
| 3 | Thornhill Trojans | 58-18 | South London Storm |
| 4 | Eccles & Salford | 20-2 | Blackbrook |
| 5 | Royal Navy | 30-28 | Waterhead |
| 6 | Siddal | 18-14 | York Acorn |
| 7 | Bradford Dudley Hill | 42-4 | Brighouse Rangers |
| 8 | The Army | 20-28 | Leigh Miners Rangers |
| 9 | Eastmoor Dragons | 28-6 | Shaw Cross Sharks |
| 10 | East Leeds | 56-0 | Warrington Wizards |
| 11 | Wath Brow Hornets | 22-26 | Oulton Raiders |
| 12 | Seaton Rangers | 50-10 | Queens |
| 13 | Heworth | 10-36 | Hull Isberg |
| 14 | Drighlington | 44-0 | Askam |
| 15 | Skirlaugh | 74-0 | Milford Marlins |
| 16 | Hull Dockers | 52-8 | Thatto Heath Crusaders |
| 17 | Dewsbury Celtic | 36-20 | Ovenden |
| 18 | Crosfields | 6-34 | West Bowling |
| 19 | Egremont Rangers | 34-18 | Oldham St Annes |
| 20 | Wigan St Patricks | 28-38 | Leeds Metro. University |
| 21 | Ince Rose Bridge | 12-35 | Widnes St Maries |
| 22 | Royal Air Force | 0-42 | East Hull |
| 23 | West Hull | 38-12 | Castleford Panthers |
| 24 | Hull Victoria | 14-44 | Castleford Lock Lane |
| 25 | Stanningley | 20-36 | Leigh East |
| 26 | Bramley Buffaloes | 12-18 | Wigan St Judes |
| 27 | Normanton Knights | 46-8 | Fife Lions |
Read more about this topic: 2007 Challenge Cup