Round 1
- (week ending 5th February)
Sees the introduction of the major amateur clubs from the National Conference League and the teams from National League 3
| Tie no | Home team | Score | Away team |
|---|---|---|---|
| 1 | Thornhill Trojans | 30-12 | Eastmoor Dragons |
| 2 | West Bowling | 15–14 | Halton Simms Cross |
| 3 | Bramley Buffaloes | 30-12 | Thatto Heath Crusaders |
| 4 | Clayton | 24-32 | Warrington Wizards |
| 5 | Royal Navy | 16-38 | Wigan St Patricks |
| 6 | Eccles & Salford | 10-12 | Hunslet Warriors |
| 7 | Leigh East | 86-10 | Askam |
| 8 | Shaw Cross Sharks | 32-22 | Oldham St Annes |
| 9 | Skirlaugh | 12-8 | Normanton Knights |
| 10 | East Leeds | 28-12 | Milford Marlins |
| 11 | Ovenden | 32-28 | Bank Quay Bulls |
| 12 | Bradford Dudley Hill | 42-12 | Gateshead Storm |
| 13 | York Acorn | 29-22 | Ince Rose Bridge |
| 14 | Royal Air Force | 13-42 | Siddal |
| 15 | Wigan St Judes | 12-33 | Kells |
| 16 | Walney Central | 18-0 | Ideal Isberg |
| 17 | East Hull | 46-0 | Lock Lane |
| 18 | Leigh Miners | 76-0 | Bransholme |
| 19 | Gloucestershire Warriors | 20-36 | Blackbrook |
| 20 | Seaton Rangers | 34-4 | Rochdale Mayfield |
| 21 | Barrow Island | 32-18 | Stanley Rangers |
| 22 | Crosfields | 20-28 | Oulton Raiders |
| 23 | Saddleworth Rangers | 33-32 | Hull Dockers |
| 24 | Widnes St Maries | 40-12 | St Albans Centurions |
| 25 | The Army | 38-18 | West Hull |
Read more about this topic: 2006 Challenge Cup