1st Round
| August 18, 1940 | |||
| SpVgg Fürth | 3 – 0 | VfB Stuttgart | |
| FC Bayern Munich | 0 – 1 | Wiener SC | |
| 1. SV Jena | 0 – 1 | TuRa Leipzig | |
| VfB Königsberg | 3 – 2 | SC Preußen Danzig | (AET) |
| BuEV Danzig | 6 – 2 | Luftwaffen SV Stettin | |
| Blau-Weiß 90 Berlin | 1 – 2 | SV Werder Bremen | |
| Hallescher FV Sportfreunde | 0 – 7 | FC Schalke 04 | |
| SV 08 Steinach | 4 – 2 | CSC 03 Kassel | |
| SV Dessau 05 | 2 – 2 | Kickers Offenbach | |
| Eimsbütteler TV | 0 – 3 | Spandauer SV | |
| Blumenthaler SV | 3 – 1 | Hamburger SV | |
| SV 07 Linden | 2 – 3 | SC Union 06 Oberschöneweide | |
| SV 07 Hildesheim | 2 – 3 | Barmbeker SG | (AET) |
| VfL Osnabrück | 2 – 5 | BSG Gelsenguß Gelsenkirchen | |
| TuS Neheim | 2 – 3 | SG Eschweiler | |
| BSG Edelstahlwerke Krefeld | 3 – 4 | Rot-Weiß Essen | |
| TuS Germania Mudersbach | 1 – 8 | Schwarz-Weiß Essen | |
| TuS Neuendorf | 1 – 2 | VfR Mannheim | |
| BC Sport Kassel | 4 – 5 | TuS 48/99 Duisburg | |
| Eintracht Frankfurt | 3 – 2 | Westfalia Herne | |
| 1. FC Kaiserslautern | 2 – 3 | Fortuna Düsseldorf | |
| SV Waldhof Mannheim | 2 – 3 | Reichsbahn TuSV Frankfurt | (AET) |
| Phönix Karlsruhe | 4 – 2 | FSV Frankfurt | |
| SpVgg Cannstatt | 0 – 7 | Wacker Wien | |
| VfR Schweinfurt 07 | 2 – 1 | Mühlheimer SV 06 | (AET) |
| Sturm Graz | 1 – 6 | 1. FC Nuremberg | |
| NSTG Vitkovice | 6 – 0 | SC Hertha Breslau | |
| VfL Stettin | 3 – 2 | PSV Chemnitz | |
| Planitzer SC | 3 – 1 | Vorwärts-Rasensport Gleiwitz | |
| VfB Waldshut | 0 – 8 | Stuttgarter Kickers | |
| NSTG Graslitz | 0 – 4 | Dresdner SC | |
| Neumeyer Nürnberg | 1 – 2 | Rapid Wien | (AET) |
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